FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_55.3510000005e806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_55.3510000005e806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38887
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC3170.8151824517190835No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC850.21858204541363438No Hit
CTTCAGCAATGCGGCTGTCTCTTATAC600.15429320852727132No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC250.003073654821.2823521
ACAGCAA250.003073654821.2823523
CTTCAGC31750.021.2823521
CGTCAGC354.0192077E-521.2823521
TACAGCA250.003073654821.2823522
TTCCGCA2900.021.2823522
TCGGCAA404.6216464E-621.2823523
CTCCAGC455.3293843E-721.2823521
TTCTGCA250.003073654821.2823522
TCCAGCA455.3293843E-721.2823522
TCAGCAA32650.021.2823523
TCTGCAA250.003073654821.2823523
CCTCAGC506.159462E-821.2823521
CTTCGGC404.6216464E-621.2823521
TCCGCAA2900.021.2823523
CTGCAGC1200.021.2823521
GTCAGCA354.0192077E-521.2823522
CTTCCGC2900.021.2823521
CTTCTGC250.003073654821.2823521
TTCGGCA404.6216464E-621.2823522
Produced by FastQC (version 0.11.2)