FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_39.3510000005e5fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_39.3510000005e5fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46718
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA1880.4024144869215292No Hit
ACTTCGTCAGCTGTCTCTTATACACAT1250.26756282375101675No Hit
ACTTCGTCAGCTCTGTCTCTTATACAC790.16909970461064258No Hit
ACTTCGTCAGCTGCTGTCTCTTATACA510.10916563209041484No Hit
ACTTCGTCAGCTCCTGTCTCTTATACA480.10274412432039043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGGT250.00310699121.2540721
CCTCGTC651.0004442E-1021.2540722
CTTGGTC250.00310699121.2540722
ACCTCGT651.0004442E-1021.2540721
TTGGTCA250.00310699121.2540723
CTCGTCA651.0004442E-1021.2540723
TTCGTCA39600.021.254073
ACGTCGT900.021.254071
GTCGTCA900.021.254073
CTTAGTC3650.021.254072
ACATCGT608.54925E-1021.254071
ACTTAGT3650.021.254071
CGTCGTC900.021.254072
ATCGTCA608.54925E-1021.254073
TTAGTCA3650.021.254073
TTTCGTC455.447946E-721.254072
CATCGTC608.54925E-1021.254072
ACTTCGT39250.021.2269951
CTTCGTC39250.021.2269952
CAGCTCT2450.020.9582468
Produced by FastQC (version 0.11.2)