FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_32.3510000005e523.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_32.3510000005e523.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87902
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC5050.5745034242679348No Hit
GGATAGTTAGCCTGTCTCTTATACACA2620.29805920229346317No Hit
GGATAGTTAGCCCCTGTCTCTTATACA1040.11831357648290142No Hit
GGATAGTTAGCCGCTGTCTCTTATACA970.11035016268116765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT404.4076005E-621.4585761
ATAGTTA73200.021.4585763
CTAGTTA4600.021.4585763
ATCGTTA4450.021.4585763
TTAGTTA2950.021.4585763
GATAGTT73050.021.4145152
GGATAGT73000.021.4144841
GGCTAGT4650.021.2278391
GATCGTT4500.021.2201482
GGATCGT4500.021.2201481
GGTTAGT3000.021.1009331
TTCATTT303.9782908E-420.9334117
TGCATTT303.9782908E-420.9334117
GTCATTT303.9782908E-420.9334117
TAAATTT303.9782908E-420.9334117
AAATTTT950.020.9334118
TGACTTT601.0713848E-920.9334117
ATGATTT405.4655193E-620.9334117
TCGGTTT405.4655193E-620.9334117
GCAGGTT303.9782908E-420.9334116
Produced by FastQC (version 0.11.2)