FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_24.3510000005e417.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_24.3510000005e417.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52837
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGCTGTCTCTTATACACA6491.2283059219864867No Hit
GTGTAGTGTGGAGGCTGTCTCTTATAC6041.143138331093741No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC2440.46179760395177627No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC2240.42394534133277817No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC2230.42205272820182826No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC2110.3993413706304294No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC2000.3785226261899805No Hit
GTGTAGTGTGGACCTGTCTCTTATACA1810.34256297670193236No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA1720.3255294585233832No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC1620.30660332721388417No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC1170.2214357363211386No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC1130.213865283797339No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC930.17601302117834094No Hit
GTGTAGTGTGGATCTGTCTCTTATACA900.17033518178549123No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC840.15897950299979183No Hit
GTGTAGTGTGGACTCTGTCTCTTATAC770.1457312110831425No Hit
GTGTAGTGTGGATACTGTCTCTTATAC760.1438385979521926No Hit
GTGTAGTGTGGAAACTGTCTCTTATAC730.13816075855934287No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC670.12680507977364347No Hit
GTGTAGTGTGGACACTGTCTCTTATAC630.11923462724984386No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGT353.907158E-521.3849121
GCGTAGT455.160946E-721.3849121
AGTAGTG303.4403204E-421.3642522
GTCGTGT2500.021.3642523
CGTAGTG455.2093674E-721.3642522
TGTGGTG303.4403204E-421.3642522
GTGGTGT303.4403204E-421.3642523
TGTCGTG2500.021.3642522
TTTAGTG353.9364895E-521.364252
TGTAGTG46650.021.364252
GGTAGTG556.9667294E-921.364252
TTAGTGT353.9364895E-521.364253
GTAGTGT47750.021.364253
GTGTAGT46500.021.361921
GTGTCGT2550.020.9656011
AGGGGTT250.003394228620.93939616
GGAGATG250.003394228620.93939610
GGAGATA303.9416936E-420.93939610
GATGGGG303.9416936E-420.93939611
GAGGCGG250.003394228620.93939611
Produced by FastQC (version 0.11.2)