FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_16.3510000005e318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_16.3510000005e318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80864
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC7700.9522160664819944No Hit
GAACACCATAGCGGCTGTCTCTTATAC4130.5107340720221607No Hit
GAACACCATAGCGCCTGTCTCTTATAC2690.33265730114760583No Hit
GAACACCATAGCGTCTGTCTCTTATAC2020.24980213692125047No Hit
GAACACCATAGCTGTCTCTTATACACA2000.2473288484368817No Hit
GAACACCATAGCAGCTGTCTCTTATAC1940.23990898298377525No Hit
GAACACCATAGCACCTGTCTCTTATAC1770.21888603086664027No Hit
GAACACCATAGCCCCTGTCTCTTATAC1650.20404629996042736No Hit
GAACACCATAGCATCTGTCTCTTATAC1610.19909972299168976No Hit
GAACACCATAGCGACTGTCTCTTATAC1520.18796992481203006No Hit
GAACACCATAGCTGCTGTCTCTTATAC1350.16694697269489514No Hit
GAACACCATAGCCGCTGTCTCTTATAC1310.1620003957261575No Hit
GAACACCATAGCTCCTGTCTCTTATAC1160.14345073209339138No Hit
GAACACCATAGCCCTGTCTCTTATACA1090.13479422239810052No Hit
GAACACCATAGCGCTGTCTCTTATACA870.10758804907004353No Hit
GAACACCATAGCAACTGTCTCTTATAC860.10635140482785913No Hit
GAACACCATAGCTTCTGTCTCTTATAC850.1051147605856747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACACC70450.021.3415261
GATCACC800.021.3415261
GTACACC900.021.3415241
GAACCCC3150.021.3415241
GACCACC3550.021.3415241
ACCACCA3550.021.3280852
AACCCCA3150.021.3280852
TACACCA900.021.3280852
ACCCCAT3150.021.3280853
ATCACCA800.021.3280852
TCACCAT800.021.3280853
AACACCA70500.021.3280852
ACACCAT71450.021.3131623
CCACCAT3600.021.0318623
TGTTGTT250.003403867620.9455816
ACTGTTT354.6266443E-520.9455817
AGCCCGG354.6266443E-520.9455810
GCGGGGC507.5498974E-820.9455811
GCGGGCG250.003403867620.9455811
TAGCGCC1000.020.945589
Produced by FastQC (version 0.11.2)