FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_15.3510000005e2fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_15.3510000005e2fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84488
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA9911.1729476375343244No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT2420.28643120916579873No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC1660.19647760628728342No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT1350.15978600511315216No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA990.11717640374964491No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT980.1159928037117697No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT880.1041568033330177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACATA250.002985841821.424851
TGGCTTA303.398757E-421.424851
TTGCATA800.021.4248491
TGTCATA1550.021.4248491
TGGAATA556.892151E-921.4248491
TGGCCTA5500.021.4248491
TGGCATA72700.021.4248471
TGGCGTA659.276846E-1121.4248471
GTCATAG1550.021.4118732
GCCTAGC5500.021.4118733
GGCCTAG5500.021.4118732
GGCTTAG303.4127236E-421.4118732
TCATAGC1550.021.4118733
GCATAGC73550.021.4118733
GCTTAGC303.4127236E-421.4118733
TGCATAG800.021.4118712
GCGTAGC659.458745E-1121.4118713
GGCATAG72850.021.3971752
TTTTTCT303.9648163E-420.94253721
GTTTTCT303.9648163E-420.94253720
Produced by FastQC (version 0.11.2)