FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_93.3510000005e0bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_93.3510000005e0bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTAAAAAAAAAAAAAAAA50.12667848999239928No Hit
ATCTCGACGCTGTCTCTTATACACATC50.12667848999239928No Hit
ATCTCGACGCTAGGGGCTGTCTCTTAT40.10134279199391943No Hit
ATCTCGACGCTAGGGCCTGTCTCTTAT40.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG3500.021.2337683
ATCTCGA3450.021.2337671
TCTCGAC3450.021.2337672
TCTAGAC250.002638026421.2337652
ATCTAGA250.002638026421.2337651
CTAGACG250.002638026421.2337653
GTGTTTT303.0887354E-420.96153818
ACGCTAT800.020.9615387
ACGCTAC1200.020.9615387
ACGCTAA453.7455357E-720.9615387
TCGACGC3550.020.9615384
TTTTTTT2350.020.96153821
TGTTTTT453.7455357E-720.96153819
GTTTTTT1250.020.96153820
GGTTTTT250.002850400520.96153819
CGCTATT403.5437388E-620.9615388
CGCTAGT303.0887354E-420.9615388
CGCTAGG604.1472958E-1020.9615388
CGCTACT303.0887354E-420.9615388
CGCTACG403.5437388E-620.9615388
Produced by FastQC (version 0.11.2)