FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_92.3510000005e092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_92.3510000005e092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17040
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCA658.367351E-1121.2686581
CGCCACT658.367351E-1121.2686583
TCGCCAC658.367351E-1121.2686582
TTAGACA709.094947E-1221.2686561
AGCCACT15000.021.2686563
TAGCCAC14700.021.2686562
TGCCACT353.866696E-521.2686563
AGACACT709.094947E-1221.2686563
TTAGCCA14700.021.2686561
TAGACAC709.094947E-1221.2686562
TTTGCCA353.866696E-521.2686561
TTGCCAC353.866696E-521.2686562
CTCTGCT557.708877E-920.9558858
CTCTGCA557.708877E-920.9558858
CTCTGAC557.708877E-920.9558858
TCTGGGA557.708877E-920.9558859
TCTGGCG557.708877E-920.9558859
TCTGGAG557.708877E-920.9558859
TCTGTAG557.708877E-920.9558859
ATCTGTC557.708877E-920.95588520
Produced by FastQC (version 0.11.2)