FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_90.3510000005e05f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_90.3510000005e05f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5460
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGAG4450.021.336452
TTCCAGA4450.021.336451
CCAGAGA4650.021.3364493
TTCCCGA604.3837645E-1021.3364491
CCCGAGA604.3837645E-1021.3364493
TCCCGAG604.3837645E-1021.3364492
GACTGGA555.2186806E-920.9449568
ACTGCGG250.003018233720.9449549
ACTGTGT353.6925994E-520.9449549
AATTTTT303.3443543E-420.94495419
ACTGAAG250.003018233720.9449549
GGATTTT303.3443543E-420.94495418
GGTTTTT504.829053E-820.94495419
CATTTTT250.003018233720.94495419
CAGAGAC4800.020.9449544
GATTTTT353.6925994E-520.94495419
GACTGTG705.456968E-1220.9449548
AGAGACT4800.020.9449545
GACTGGT404.0595114E-620.9449548
GACTGGG900.020.9449548
Produced by FastQC (version 0.11.2)