FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_89.3510000005e035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_89.3510000005e035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8093
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC510.6301742246385766No Hit
TCAACCGATAGGGGCTGTCTCTTATAC170.21005807487952552No Hit
TCAACCGATAGGCTGTCTCTTCTACAC170.21005807487952552No Hit
TCAACCGATAGGCCTGTCTCTTATACA120.1482762881502533No Hit
TCAACCGATAGGGCTGTCTCTTATACA110.13591993080439888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGA7250.021.1125012
TCAACCG7250.021.1125011
AACCGAT7300.021.11253
CACCGAT250.00300432321.11253
CCACCGA250.00300432321.11252
TCCACCG250.00300432321.11251
CGATAGG8050.020.9813676
TAGGAGC250.003116676620.9813679
AATTTTT353.9445204E-520.98136719
ATAGGTG404.430771E-620.9813678
ATAGGTA709.094947E-1220.9813678
TTATTTT250.003116676620.98136718
ATAGGGT657.6397555E-1120.9813678
ATAGGGA404.430771E-620.9813678
TTTTTTT4400.020.98136721
ATAGGCC404.430771E-620.9813678
ATAGGAC250.003116676620.9813678
TTCTGTC353.9445204E-520.98136721
GTTTTTT1950.020.98136720
GGTTTTT751.8189894E-1220.98136719
Produced by FastQC (version 0.11.2)