FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_87.3510000005e00e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_87.3510000005e00e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA120.3091190108191654No Hit
ACTTCGTCAGCTGTCTCTTATACACAT80.20607934054611027No Hit
ACTTCGTCAGCTCTGTCTCTTATACAC50.1287995878413189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA3500.021.4800013
ACTTCGT3400.021.4800011
CTTCGTC3400.021.4800012
CAGCTCG303.1177263E-420.922088
CAGCTCC250.002874814220.922088
CAGCTAG604.2018655E-1020.922088
CAGCTAC250.002874814220.922088
CGTCAGC3750.020.922085
AGCTAGC303.1177263E-420.922089
TTTTTTT2250.020.9220821
GTTTTTT900.020.9220820
GGTTTTT250.002874814220.9220819
TCAGCTG1250.020.922087
TCGTCAG3750.020.922084
GTCAGCT3850.020.922086
AGCTGGG250.002874814220.922089
CTTTTTT503.9619408E-820.9220820
CAGCTGG654.1836756E-1120.922088
TGTTTTT353.355511E-520.92207719
TCAGCTT703.6379788E-1220.9220777
Produced by FastQC (version 0.11.2)