FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_86.3510000005dfe6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_86.3510000005dfe6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA430.7874015748031495No Hit
TGTTGCCGAACAGCTGTCTCTTATACA110.20142830983336385No Hit
TGTTGCCGAACACCTGTCTCTTATACA90.16480498077275224No Hit
TGTTGCCGAACAGGGGGGGGTTTTTTT60.10986998718183483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCC4800.021.5094341
TGGTGCC250.002576530221.5094341
GTTGCCG4800.021.5094342
GGTGCCG250.002576530221.5094342
TTGCCGA4800.021.5094343
GTGCCGA250.002576530221.5094343
TTTTTTT3900.020.91743321
GGGTTTT353.730297E-520.9174318
AACAAGG353.730297E-520.917439
AATTTTT353.730297E-520.9174319
TGCCGAA5350.020.917434
TGTTTTT605.693437E-1020.9174319
GTTTTTT2100.020.9174320
GGTTTTT707.2759576E-1220.9174319
ACTTTTT250.003041368420.9174319
TCTGTCT250.003041368420.9174321
CGAACAT1050.020.917437
CGAACAG2050.020.917437
CGAACAC800.020.917437
CGAACAA1050.020.917437
Produced by FastQC (version 0.11.2)