FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_85.3510000005dfbf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_85.3510000005dfbf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8223
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA550.6688556487899794No Hit
CTCTAGTACCGACCTGTCTCTTATACA110.13377112975799585No Hit
CTCTAGTACCGAGCCTGTCTCTTATAC110.13377112975799585No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC100.12161011796181442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT6900.021.3478261
CTCTCGT605.529728E-1021.3478241
TCTAGTA6950.021.216052
CTAGTAC7200.021.216053
TCTCGTA606.002665E-1021.2160472
CTCGTAC606.002665E-1021.2160473
TACCGAT1750.020.957327
TACCGAG2750.020.957327
GTGTTTT250.003142538220.95731718
GGGTTTT250.003142538220.95731718
AATTTTT556.319169E-920.95731719
GTACCGC303.542482E-420.9573176
GTACCGA7900.020.9573176
TGTTTTT900.020.95731719
GGATTTT303.542482E-420.95731718
GGTTTTT800.020.95731719
ACCGATT303.542482E-420.9573178
ACCGATG505.6561476E-820.9573178
ACCGATC404.49254E-620.9573178
ACCGATA556.319169E-920.9573178
Produced by FastQC (version 0.11.2)