FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_84.3510000005df96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_84.3510000005df96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA510.7453960830166617No Hit
CAGAACGACAGTGTCTGTCTCTTATAC110.1607717041800643No Hit
CAGAACGACAGTGGCTGTCTCTTATAC100.14615609470914936No Hit
CAGAACGACAGTAGCTGTCTCTTATAC90.13154048523823444No Hit
CAGAACGACAGTACCTGTCTCTTATAC90.13154048523823444No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG303.1201344E-421.268661
GCACGAC303.1201344E-421.268663
GACCGAC303.1201344E-421.268663
CAGACCG303.1201344E-421.268661
AGCACGA303.1201344E-421.268662
AGACCGA303.1201344E-421.268662
GAACGAC6000.021.2686563
AGAACGA5900.021.2686562
CAGAACG5900.021.2686561
CGACAGT6600.020.9558836
ACAGTGG1550.020.9558838
ACAGTGA303.4566142E-420.9558838
ACAGTCC353.8623213E-520.9558838
GACAGTT1200.020.9558837
AACGACA6200.020.9558834
TGTTTTT353.8623213E-520.95588319
CATTTTT353.8623213E-520.95588319
CACGACA303.4566142E-420.9558834
ACCGACA303.4566142E-420.9558834
GCGTTTT303.4566142E-420.95588318
Produced by FastQC (version 0.11.2)