FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_83.3510000005df7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_83.3510000005df7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18686
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA1901.0168040244032965No Hit
CACAGAAGGACTGCTGTCTCTTATACA290.1551964037247137No Hit
CACAGAAGGACCTGTCTCTTCTACCCA220.11773520282564486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA950.021.1951241
ACCGAAG950.021.1951242
CCGAAGG950.021.1951243
TAGAAGG303.508905E-421.1951223
ACAGAAG16500.021.1951222
ATAGAAG303.508905E-421.1951222
CATAGAA303.508905E-421.1951221
CAGAAGG16750.021.1951223
CACAGAA16500.021.1951221
TCAGAAG250.003086874521.195122
CTCAGAA250.003086874521.195121
GGACTCG1650.020.967838
TTGTTTT455.8071055E-720.96782918
ACTGCGG303.7762016E-420.96782910
GTGTTTT701.2732926E-1120.96782918
AGCTTTT405.022266E-620.96782918
ACTTGTG354.350137E-520.96782910
AATTTTT1200.020.96782919
AGAAGGA17350.020.9678294
AGGACTT3900.020.9678297
Produced by FastQC (version 0.11.2)