FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_81.3510000005df39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_81.3510000005df39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9934
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA270.27179383933964163No Hit
AACGGAATGGCTGTCTCTTATACACAT230.23152808536339842No Hit
AACGGAATGGCTGCTGTCTCTTATACA220.22146164686933761No Hit
AACGGAATGGCTCTGTCTCTTATACAC200.20132876988121604No Hit
AACGGAATGGCTCCTGTCTCTTATACA170.17112945439903363No Hit
AACGGAATGGCTACTGTCTCTTATACA110.11073082343466881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAA9150.021.56251
ACGGAAT9150.021.56252
CGGAATG9200.021.5624983
AATTTTT658.913048E-1120.90909219
TGATTTT404.7500525E-620.90909218
ATGGCTT2450.020.9090927
TGTTTTT658.913048E-1120.90909219
ATGGCTC1750.020.9090927
ATGGCTA1750.020.9090927
GTATTTT303.6734607E-420.90909218
CATTTTT303.6734607E-420.90909219
GGCTGGA354.177439E-520.9090929
CCTTTTT404.7500525E-620.90909219
TAATTTT303.6734607E-420.90909218
GGCTTGG303.6734607E-420.9090929
GGCTTGA303.6734607E-420.9090929
GGCTCAG354.177439E-520.9090929
GATTTTT950.020.90909219
GGCTAGG303.6734607E-420.9090929
CGGTTTT404.7500525E-620.90909218
Produced by FastQC (version 0.11.2)