FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_80.3510000005df1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_80.3510000005df1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15525
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC650.4186795491143317No Hit
GGATAGTTAGCCTGTCTCTTATACACA290.18679549114331723No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC160.10305958132045087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGT555.487891E-921.5382081
TTAGTTA555.487891E-921.5382083
GGATAGT12950.021.5382061
ATAGTTA12900.021.5382063
GATAGTT12950.021.5382062
GATCGTT1000.021.5382042
GGATCGT1000.021.5382041
CTAGTTA504.8945367E-821.5382043
ATCGTTA1000.021.5382043
AGCCCGG701.2732926E-1120.9129059
GGGTTTT850.020.91290518
TTATTTT354.3763474E-520.91290518
TAGCCTT701.2732926E-1120.9129058
TAGCCTG1400.020.9129058
TAGCCTC701.2732926E-1120.9129058
TAGCCTA701.2732926E-1120.9129058
TAGCCGC701.2732926E-1120.9129058
TAGCCGA1150.020.9129058
TAGCCCA557.756171E-920.9129058
TAGCCAG1150.020.9129058
Produced by FastQC (version 0.11.2)