FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_78.3510000005deda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_78.3510000005deda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC300.7600709399543958No Hit
AGTGTGACGAACCTGTCTCTTCTACAC260.6587281479604763No Hit
AGTGTGACGAACGGCTGTCTCTTATAC70.177349885989359No Hit
AGTGTGACGAACGCTGTCTCTTATACA60.15201418799087915No Hit
AGTGTGACGAACAGCTGTCTCTTATAC50.12667848999239928No Hit
AGTGTGACGAACACCTGTCTCTTATAC50.12667848999239928No Hit
AGTGTGACGAACGGGGGCGGTTTTTTT40.10134279199391943No Hit
AGTGTGACGAACGGGGGGGCTTTTTTT40.10134279199391943No Hit
AGTGTGACGAACGGGCGGGGTTTTTTT40.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT250.002820193521.018
TTTTTTT2450.021.021
GTATTTT250.002820193521.018
GAACGGG353.275441E-521.09
GAACGGC353.275441E-521.09
GTTTTTT1200.021.020
GGTTTTT604.0563464E-1021.019
CGAACGT250.002820193521.08
CGAACGG1000.021.08
CGAACGC453.6797974E-721.08
CGAACGA403.4876957E-621.08
CGAACCG250.002820193521.08
CGAACAT250.002820193521.08
CGAACAG353.275441E-521.08
TATTTTT403.4876957E-621.019
GACGAAC3900.021.06
GTGACGA3750.021.04
ATTTTTT800.021.020
ACGAACT453.6797974E-721.07
ACGAACG2100.021.07
Produced by FastQC (version 0.11.2)