FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_75.3510000005de7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_75.3510000005de7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCAACGAGCTGTCTCTTATACA50.12667848999239928No Hit
TTCGACCAACGCTGTCTCTTATACACA50.12667848999239928No Hit
TTCGACCAACGACTGTCTCTTATACAC50.12667848999239928No Hit
TTCGACCAACGACTGTCTCTTCTACAC40.10134279199391943No Hit
TTCGACCAACGACCTGTCTCTTATACA40.10134279199391943No Hit
TTCGACCAACGAGCGGCGGGTTTTTTT40.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCA3150.021.4736842
TTCGCCC402.8379109E-621.4736841
CGCCCAA402.8379109E-621.4736843
TTCGACC3150.021.4736841
TCGCCCA402.8379109E-621.4736842
CGACCAA3250.021.4736843
CCAACGA3850.020.9230796
GCCCAAC403.6007877E-620.9230774
AACGATC250.00288098520.9230778
AACGAGT303.1273896E-420.9230778
AACGAGG750.020.9230778
AACGAGC503.9968654E-820.9230778
AACGACT250.00288098520.9230778
AACGACG453.8125836E-720.9230778
AACGAAG250.00288098520.9230778
ACCAACG3450.020.9230775
TCTGTCT303.1273896E-420.92307721
CCCAACG403.6007877E-620.9230775
ACGAGGG303.1273896E-420.9230779
CCTTTTT303.1273896E-420.92307719
Produced by FastQC (version 0.11.2)