FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_74.3510000005de54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_74.3510000005de54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC902.24271118863693No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC491.221031647146773No Hit
ACTGTCTCTGTCTCTTATACACATCTC491.221031647146773No Hit
ACTGTCTCTGTCTCTTATACCCATCTC120.299028158484924No Hit
ACTGTCTGTGTCCCTGTCTCTTATACA110.27410914527784697No Hit
ACTGTCTGTGTCTGTCTCTTATACACA90.224271118863693No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTG3550.021.4615382
GTGTCTT403.639394E-620.9250018
GTGTCCT604.3655746E-1020.9250018
GTGTCAT303.1460513E-420.9250018
GTGTCAG303.1460513E-420.9250018
TTTTTTT2450.020.92500121
TGTTTTT303.1460513E-420.92500119
GTTTTTT1200.020.92500120
GTCTGTG3900.020.9250014
TCTGTGT3900.020.9250015
CTGTGTC3900.020.9250016
ATTTTTT604.3655746E-1020.92500120
AGTTTTT303.1460513E-420.92500119
TGTGTCT800.020.9250017
TGTGTCC1150.020.9250017
TGGTTTT250.002892886520.92518
GTGTCGG504.0648956E-820.9258
GTGTCGC250.002892886520.9258
GTGTCCG353.3972257E-520.9258
TGTCCTG504.0648956E-820.9259
Produced by FastQC (version 0.11.2)