FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_73.3510000005de3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_73.3510000005de3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3947
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC130.32936407398023815No Hit
CTTGGTTCGTTGCTGTCTCTTCTACAC60.15201418799087915No Hit
CTTGGTTCGTTGGCTGTCTCTTATACA50.12667848999239928No Hit
CTTGGTTCGTTGTCTGTCTCTTATACA40.10134279199391943No Hit
CTTGGTTCGTTGGGGGGGGATTTTTTT40.10134279199391943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG3550.021.4736843
CTTGGTT3400.021.4736841
TTGGTTC3400.021.4736842
GGTTCGT3850.020.9230794
TTCGTTG3750.020.9230776
GGTTTTT250.00288098520.92307719
CATTTTT403.6007877E-620.92307719
CCTTTTT250.00288098520.92307719
TCGTTGT1000.020.9230777
TCGTTGG1150.020.9230777
TCGTTGC950.020.9230777
GTTGGGG250.00288098520.9230779
CGTTGTG403.6007877E-620.9230778
CGTTGTA250.00288098520.9230778
CGTTGGT250.00288098520.9230778
CGTTGGG453.8125836E-720.9230778
CGTTGGC250.00288098520.9230778
CGTTGCT303.1273896E-420.9230778
CGTTGCG303.1273896E-420.9230778
CGTTGCA303.1273896E-420.9230778
Produced by FastQC (version 0.11.2)