FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_71.3510000005ddf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_71.3510000005ddf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8290
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA881.0615199034981906No Hit
ATCTGCTATCCTGTCTCTTATACACAT220.26537997587454765No Hit
ATCTGCTATCCGGCTGTCTCTTATACA130.15681544028950542No Hit
ATCTGCTATCCGGGGGGGGGTTTTTTT100.12062726176115801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCTA7250.021.4472052
CTGCTAT7550.021.4472053
ATCTGCT7250.021.4472051
TGGTTTT354.0400268E-520.92727518
TCCGGTG354.0400268E-520.9272759
TCCGGGT354.0400268E-520.9272759
TCCGGGG709.094947E-1220.9272759
TATCCGG3000.020.9272757
TATCCGA1500.020.9272757
TATTTTT709.094947E-1220.92727519
ATCCGTC354.0400268E-520.9272758
ATCCGGA354.0400268E-520.9272758
ATCCGCG709.094947E-1220.9272758
ATCCGCA354.0400268E-520.9272758
ATTTTTT2350.020.92727520
CTATCCG8050.020.9272756
GTGTTTT303.579632E-420.92727318
AATTTTT556.4410415E-920.92727319
CGGGGAG250.003170468120.92727311
TGTTTTT455.1253846E-720.92727319
Produced by FastQC (version 0.11.2)