FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_67.3510000005dd7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_67.3510000005dd7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11184
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAGCGCATTCTGTCTCTTATACAC180.1609442060085837No Hit
TCAGAGCCCATTCACCTGTCTCTTATA130.11623748211731044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGCG9950.021.3287682
TCAGCGC505.088623E-821.3287681
TCAGAGC9950.021.3287681
CGAGCGC353.6295613E-521.3287683
CCGAGCG353.6295613E-521.3287682
AGCGCGC505.088623E-821.3287683
CAGCGCG505.088623E-821.3287682
TCCGAGC353.6295613E-521.3287681
AGAGCGC10000.021.3287663
TGTTTTT1100.020.9461919
GATTTTT1100.020.9461919
CGCATTG4200.020.946197
CGCATTC2650.020.946197
GCATTTG557.039489E-920.946198
GCATTGC1100.020.946198
GCATTAT557.039489E-920.946198
GCATTAC557.039489E-920.946198
AGTTTTT1050.020.9461919
CGTTTTT557.039489E-920.9461919
TGGTTTT303.6704796E-420.94618818
Produced by FastQC (version 0.11.2)