FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_66.3510000005dd61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_66.3510000005dd61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9869
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCTGTCTCTTATACACA530.5370351606039113No Hit
GCATTGGATACCCTGTCTCTTATACAC360.36477859965548687No Hit
GCATTGGATACCGGCTGTCTCTTATAC240.24318573310365793No Hit
GCATTGGATACCATCTGTCTCTTATAC210.21278751646570068No Hit
GCATTGGATACCGTCTGTCTCTTATAC200.2026547775863816No Hit
GCATTGGATACCACCTGTCTCTTATAC200.2026547775863816No Hit
GCATTGGATACCGCCTGTCTCTTATAC180.18238929982774343No Hit
GCATTGGATACCTGCTGTCTCTTATAC180.18238929982774343No Hit
GCATTGGATACCCGCTGTCTCTTATAC160.16212382206910528No Hit
GCATTGGATACCGCTGTCTCTTATACA150.15199108318978619No Hit
GCATTGGATACCCCCTGTCTCTTATAC140.14185834431046712No Hit
GCATTGGATACCCTCTGTCTCTTATAC120.12159286655182897No Hit
GCATTGGATACCTACTGTCTCTTATAC120.12159286655182897No Hit
GCATTGGATACCAGCTGTCTCTTATAC120.12159286655182897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGA8900.021.468752
GCCTTGG504.5909474E-821.468751
ATTGGAT9000.021.468753
CTTGGAT504.5909474E-821.468753
GCATTGG8900.021.468751
TTGTTTT250.0032178620.92385918
ACCGGGG354.1519364E-520.92385910
ACCGGCG250.0032178620.92385910
CAGTTTT250.0032178620.92385918
GATACCG3850.020.9238597
GATACCC2600.020.9238597
GATACCA1600.020.9238597
TGATTTT250.0032178620.92385918
TGTTTTT800.020.92385919
GGATACC9850.020.9238596
TACCGTG250.0032178620.9238599
TACCGTA354.1519364E-520.9238599
TACCGGG658.731149E-1120.9238599
TACCGCC250.0032178620.9238599
TACCGAA250.0032178620.9238599
Produced by FastQC (version 0.11.2)