FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_64.3510000005dd11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_64.3510000005dd11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC1491.3243267265131988No Hit
GAACACCATAGCGGCTGTCTCTTATAC970.8621455870589281No Hit
GAACACCATAGCGCCTGTCTCTTATAC450.3999644476046574No Hit
GAACACCATAGCGTCTGTCTCTTATAC420.3733001510976802No Hit
GAACACCATAGCCTGTCTCTTCTACAC410.36441205226202117No Hit
GAACACCATAGCACCTGTCTCTTATAC390.34663585459070306No Hit
GAACACCATAGCAGCTGTCTCTTATAC390.34663585459070306No Hit
GAACACCATAGCTGCTGTCTCTTATAC380.337747755755044No Hit
GAACACCATAGCTGTCTCTTATACACA340.3021953604124078No Hit
GAACACCATAGCCCCTGTCTCTTATAC320.2844191627410897No Hit
GAACACCATAGCATCTGTCTCTTATAC300.2666429650697716No Hit
GAACACCATAGCGACTGTCTCTTATAC270.23997866856279443No Hit
GAACACCATAGCTTCTGTCTCTTATAC230.20442627322015822No Hit
GAACACCATAGCCGCTGTCTCTTATAC180.15998577904186295No Hit
GAACACCATAGCTCCTGTCTCTTATAC180.15998577904186295No Hit
GAACACCATAGCCCTGTCTCTTATACA170.1510976802062039No Hit
GAACACCATAGCTACTGTCTCTTATAC170.1510976802062039No Hit
GAACACCATAGCAACTGTCTCTTATAC170.1510976802062039No Hit
GAACACCATAGCGCTGTCTCTTATACA160.14220958137054485No Hit
GAACACCATAGCCTCTGTCTCTTATAC160.14220958137054485No Hit
GAACACCATAGCCACTGTCTCTTATAC130.11554528486356769No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACCA404.074942E-621.3257922
ACACCAT9850.021.3257923
AACCCCA454.5638444E-721.3257922
CCACCAT404.074942E-621.3257923
GAACCCC454.5638444E-721.3257921
GACCACC404.074942E-621.3257921
GAACACC9750.021.325791
AACACCA9750.021.325792
CATAGCG5050.020.9466697
TAGCGGT455.443071E-720.9466679
TAGCGGG751.8189894E-1220.9466679
TAGCGGC608.021743E-1020.9466679
TAGCGGA354.186308E-520.9466679
TAGCGCG701.0913936E-1120.9466679
TAGCGCC250.00322611820.9466679
TAGCGAG354.186308E-520.9466679
GTGTTTT354.186308E-520.94666718
GGGTTTT404.773945E-620.94666718
AATTTTT557.05586E-920.94666719
ACCATAG10750.020.9466675
Produced by FastQC (version 0.11.2)