FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_6.3510000005d5e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_6.3510000005d5e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26187
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA3291.256348569901096No Hit
CTGATCGTAGCACCTGTCTCTTATACA1010.38568755489364953No Hit
CTGATCGTAGCAGCTGTCTCTTATACA720.274945583686562No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT310.11837934853171422No Hit
CTGATCGTAGCATCTGTCTCTTATACA300.11456065986940084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT24050.021.1737462
GATCGTA24600.021.1737463
CTGATCG24050.021.1737461
CGGATCG354.11391E-521.1737441
GCTCGTA800.021.1737443
TGCTCGT800.021.1737442
CTGCTCG800.021.1737441
TAGCATG2100.020.9713218
TAGCACC651.10958354E-1020.9713218
CCTTTTT651.10958354E-1020.97132119
AGCACGG751.8189894E-1220.9713219
AGGGGGG303.8289302E-420.9713212
GTGTTTT950.020.9713218
GCCTTTT303.8289302E-420.9713218
CAGTTTT250.003319619220.9713218
TGCGCTT250.003319619220.9713216
GCAAGGG250.003319619220.9713210
TATACAC354.4323002E-520.9713220
AATTTTT1000.020.9713219
ACGTTTT405.145701E-620.9713218
Produced by FastQC (version 0.11.2)