FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_59.3510000005dc7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_59.3510000005dc7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA220.3215434083601286No Hit
AAGTGCCTCAGTGCTGTCTCTTATACA120.17538731365097923No Hit
AAGTGCCTCAGTAGCTGTCTCTTATAC90.13154048523823444No Hit
AAGTGCCTCAGTCCTGTCTCTTATACA70.10230926629640456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGCC6150.021.5454561
GTGCCTC6250.021.373093
AGTGCCT6300.021.0324692
CTCAGTG2150.020.9117667
GGGTTTT250.003126339820.91176418
CAGTCTA303.5062307E-420.9117649
CAGTTTA250.003126339820.9117649
CAGTCGG250.003126339820.9117649
GCCTCAG6400.020.9117645
AATTTTT353.925681E-520.91176419
TTTTTTT4400.020.91176421
TGTTTTT303.5062307E-420.91176419
GGATTTT353.925681E-520.91176418
GGTTTTT606.566552E-1020.91176419
GTCTGTC250.003126339820.91176420
CATTTTT404.384665E-620.91176419
TCAGTTT657.2759576E-1120.9117648
TCAGTTG454.8819857E-720.9117648
TCAGTTC250.003126339820.9117648
TCAGTTA250.003126339820.9117648
Produced by FastQC (version 0.11.2)