FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_58.3510000005dc62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_58.3510000005dc62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4013
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC541.345626713182158No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC340.847246449040618No Hit
ACGTCTCTGCCTGTCTCTTATACACAT230.5731373037627709No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT120.299028158484924No Hit
ACGTATCTGCATCTGTCTCTTCTACAC100.24919013207076998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC3550.021.2278481
GTATCTG250.002654980621.2278483
ACGTATC250.002654980621.2278481
GTCTCTG3550.021.2278483
CGTATCT250.002654980621.2278482
CGTCTCT3550.021.2278482
GTTTTTT1450.020.96250220
CTCTGCA3700.020.96255
TGCATTA403.5831763E-620.96258
TGCATGG654.3655746E-1120.96258
TGCATGC303.1081378E-420.96258
TGCATGA353.3505123E-520.96258
TGCATCT453.798432E-720.96258
TGCATCG353.3505123E-520.96258
TGCATAG303.1081378E-420.96258
AATTTTT403.5831763E-620.962519
CATCTGT250.00286294320.962510
TGTTTTT503.988316E-820.962519
GGTTTTT303.1081378E-420.962519
TCTGCAT3950.020.96256
Produced by FastQC (version 0.11.2)