FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_57.3510000005dc49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_57.3510000005dc49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8224
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTACGTAGGCTGTCTCTTATACA841.0214007782101167No Hit
TACGGTACGTAGCCTGTCTCTTATACA430.5228599221789882No Hit
TACGGTACGTAGCTGTCTCTTATACAC320.38910505836575876No Hit
TACGGTACGTAGACTGTCTCTTATACA300.3647859922178988No Hit
TACGGTACGTAGTCTGTCTCTTATACA290.3526264591439689No Hit
TACGGTACGTAGGGCTGTCTCTTATAC160.19455252918287938No Hit
TACGGTACGTAGCTGTCTCTTCTACAC150.1823929961089494No Hit
TACGGTACGTAGACCTGTCTCTTATAC110.13375486381322957No Hit
TACGGTACGTAGGGGCTGTCTCTTATA100.12159533073929961No Hit
TACGGTACGTAGGCTGTCTCTTATCCC90.10943579766536965No Hit
TACGGTACGTAGAGCTGTCTCTTATAC90.10943579766536965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG7700.021.453
TACGGTA7600.021.4499991
ACGGTAC7600.021.4499992
GTAGATG250.003169200420.926839
GTAGACG250.003169200420.926839
GGGTTTT354.036724E-520.9268318
AATTTTT354.036724E-520.9268319
TTTCTGT250.003169200420.9268321
GGTTTTT900.020.9268319
ACGTAGG2800.020.926837
ACGTAGC2000.020.926837
ACGTAGA1800.020.926837
CGTAGTT505.7440047E-820.926838
CGTAGTG505.7440047E-820.926838
CGTAGTC303.5775185E-420.926838
CGTAGGT607.166818E-1020.926838
CGTAGGG1000.020.926838
CGTAGGC505.7440047E-820.926838
CGTAGGA709.094947E-1220.926838
CGTAGCT455.1182906E-720.926838
Produced by FastQC (version 0.11.2)