FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_56.3510000005dc2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_56.3510000005dc2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8421
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC1962.3275145469659186No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC630.7481296758104738No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC490.5818786367414797No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC320.38000237501484385No Hit
ACAGTGCTCATGGCTGTCTCTTATACA180.21375133594584966No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC170.20187626172663578No Hit
ACAGTGCTCCTGTCTCTTATACACATC150.17812611328820804No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC150.17812611328820804No Hit
ACCGTGCTCATGCTGTCTCTTCTACAC130.15437596484978033No Hit
ACAGTGCTCATGGGGGGCTGTCTCTTA90.10687566797292483No Hit
ACAGTGCTCATGCCCTGTCTCTTATAC90.10687566797292483No Hit
ACAGTGCTCATGGGGGGGGGTTTTTTT90.10687566797292483No Hit
ACAGTGCTCATGCGCTGTCTCTTATAC90.10687566797292483No Hit
ACAGTGCTCATGGGGGGGCGTTTTTTT90.10687566797292483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCT604.947651E-1021.5521492
ACCGTGC604.947651E-1021.5521491
AGTGCTC7300.021.5521493
CGTGCTC604.947651E-1021.5521493
CAGTGCT7300.021.5521492
ACAGTGC7250.021.5521471
AATTTTT303.6065732E-420.91071519
CATTTTT607.3123374E-1020.91071519
ACTTTTT303.6065732E-420.91071519
TCATGTT303.6065732E-420.9107158
TCATGTG607.3123374E-1020.9107158
TCATGTA303.6065732E-420.9107158
ATGCTGT303.6065732E-420.91071510
TCATGGT709.094947E-1220.9107158
TCATGGG1700.020.9107158
TCATGGC900.020.9107158
TCATGGA607.3123374E-1020.9107158
GATTTTT709.094947E-1220.91071519
CATGGTG354.0764735E-520.9107159
CATGGGT455.1886855E-720.9107159
Produced by FastQC (version 0.11.2)