FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_53.3510000005dbb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_53.3510000005dbb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC110.88No Hit
GAGCTACGTCTAGGAGCTGTCTCTTAT40.32No Hit
GAGCTACGTCTCTGTCTCTTATACACA40.32No Hit
GAGCTACGTCTAAGGGCTGTCTCTTAT30.24No Hit
GAGCTACGTCTAGAGGCTGTCTCTTAT30.24No Hit
GAGCTACGTCTGTCTCTTATACACCTC30.24No Hit
GAGCTACGTCTAGACTGTCTCTTATAC30.24No Hit
GAGCTACGTCTAACCTGTCTCTTATAC30.24No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC30.24No Hit
GAGCTACGTCTAAGGGGGGATTTTTTT20.16No Hit
GAGCTACGTCTAGCTGGCCGTTTTTTT20.16No Hit
GAGCTACGTCTAGGTTTGGCCTGTCTC20.16No Hit
GAGCTACGTCTAGTAAGGAATTTTTTT20.16No Hit
GAGCTACGTCTAGGCCTGTCTCTTATA20.16No Hit
GAGCTACGTCTAGGTCTGTCTCTTATA20.16No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT20.16No Hit
GAGCTACGTCTACTGTCTCTTATACAC20.16No Hit
GAGCTACGTCTAAGCCGAAGTTTTTTT20.16No Hit
GAGCTACGTCTAGCGCTGTCTCTTATA20.16No Hit
GAGCTACGTCTACGTCTGTCTCTTATA20.16No Hit
GAGCTACGTCTATTGGGCCTGTCTCTT20.16No Hit
GAGCTACGTCTAGTGTCTGTCTCTTAT20.16No Hit
GAGCTACGTCTACCCGCAAGTTTTTTT20.16No Hit
GAGCTACGTCTAGTTGCTGTCTCTTAT20.16No Hit
GAGCTACGTCTAGGGGTTTGTTTTTTT20.16No Hit
GAGCTACGTCTAGGCGCTGTCTCTTAT20.16No Hit
GAGCTACGTCTAGGGGTGGGTTTTTTT20.16No Hit
GAGCTACGTCTATAGTCTGTCTCTTAT20.16No Hit
GAGCTACGTCTATGAGCGGGTTTTTTT20.16No Hit
GAGCTACGTCTAGGAAGGTATTTTTTT20.16No Hit
GAGCTACGTCTAGGGCCTGTCTCTTAT20.16No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACG1200.021.0000022
TTTTTTT900.021.00000221
GAGCTAC1200.021.0000021
GCTACGT1200.021.0000023
CTACGTC1200.021.0000024
CGTCTAT301.6740938E-421.0000027
CGTCTAA301.6740938E-421.0000027
CTTTTTT301.6740938E-421.00000220
ACGTCTA1150.021.06
GTTTTTT401.1890661E-621.020
TACGTCT1250.021.05
CGTCTAG401.1890661E-621.07
GTCTAGG250.001853412620.9999988
Produced by FastQC (version 0.11.2)