FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_49.3510000005db4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_49.3510000005db4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11185
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT670.5990165400089406No Hit
GTGTCTGCTCCTGTCTCTTATCCACAT220.19669199821189093No Hit
GTGTCTGCTCATGGGCTGTCTCTTATA160.1430487259722843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCT950.021.3287683
GTGTATG950.021.3287681
GTGTCTG9450.021.3287661
TGTCTGC9450.021.3287662
GTCTGCT9800.021.3287663
GTTTTTT3300.020.9461920
TCATGGG557.039489E-920.946199
CTCATGG1700.020.946198
CTCATGA850.020.946198
GCTCATT2200.020.946197
GCTCATG4400.020.946197
GCTCATC2200.020.946197
CGTTTTT557.039489E-920.9461919
TGGTTTT250.003224738920.94618818
GTGTTTT303.6704796E-420.94618818
CAGTTTT250.003224738920.94618818
TATGCTC950.020.9461884
TGATTTT404.7684407E-620.94618818
TGTTTTT607.9853635E-1020.94618819
CAATTTT404.7684407E-620.94618818
Produced by FastQC (version 0.11.2)