FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_46.3510000005dade.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_46.3510000005dade.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45599
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA2940.6447509813811706No Hit
TACTGCAAGGAGGCTGTCTCTTATACA1070.23465426873396344No Hit
TACTGCAAGGAGCTGTCTCTTATACAC520.11403758854360842No Hit
TACTGCAAGGAGCCTGTCTCTTATACA470.10307243579903069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCA353.807329E-521.4454461
ATGCAAG353.807329E-521.4454463
AATGCAA353.807329E-521.4454462
CTGCAAG41650.021.4454463
CTACAAG556.6029315E-921.4454443
ACTGCAA40900.021.4454442
TTGCAAG950.021.4454443
TTCTGCA751.8189894E-1221.4454441
CTCCAAG607.6215656E-1021.4454443
ACTCCAA607.6215656E-1021.4454442
TATTGCA950.021.4454441
TCTGCAA751.8189894E-1221.4454442
ATTGCAA950.021.4454442
TACTGCA40900.021.4454441
TACTACA556.6029315E-921.4454441
ACTACAA556.6029315E-921.4454442
GGAGAGT558.767529E-920.9275539
GGAGAGA558.767529E-920.9275539
GGAGACA405.3836375E-620.9275539
GAGCGAG456.3194784E-720.92755310
Produced by FastQC (version 0.11.2)