FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_45.3510000005dac1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_45.3510000005dac1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15527
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGACGCTAGGGCTGTCTCTTATA160.10304630643395375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA13550.021.2352941
TCTAGAC556.4846972E-921.2352942
ATCTAGA556.4846972E-921.2352941
TCTCGAC13550.021.2352942
CTCGACG13900.021.2352923
TATCGAC303.4266457E-421.2352922
ATATCGA303.4266457E-421.2352921
ATCGACG303.4266457E-421.2352923
CGCTATG1350.020.9612928
CGCTAGC1350.020.9612928
GATTTTT1050.020.96129219
AGTTTTT1050.020.96129219
TGGTTTT354.2990476E-520.9612918
GGGTTTT506.556729E-820.9612918
AGCTTTT303.744058E-420.9612918
ACGCTAG5500.020.961297
ACGCTAA2300.020.961297
TCGACGC14600.020.961294
GCTAGTT506.556729E-820.961299
GCTAGTG455.6926547E-720.961299
Produced by FastQC (version 0.11.2)