FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_43.3510000005da71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_43.3510000005da71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27172
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA3461.2733696452230236No Hit
CTAGCTGGATGTCTGTCTCTTATACAC940.34594435448255556No Hit
CTAGCTGGATCTGTCTCTTATACACAT500.18401295451199765No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA370.13616958633887827No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA280.10304725452671869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCTG250.003115273821.2048421
CTTGCTG608.3127816E-1021.2048421
CTAGATG900.021.2048421
CTCGCTG850.021.2048421
TGCTGGA608.5128704E-1021.1654283
AGATGGA900.021.1654283
TAGATGG900.021.1654282
TTGCTGG608.5128704E-1021.1654282
CAGCTGG250.003149227721.1654282
CGCTGGA850.021.1654263
TAGCTGG24000.021.1654262
TCGCTGG850.021.1654262
AGCTGGA24400.021.1654263
CTAGCTG24000.021.1606661
GGATGTA4500.020.9705357
TCCTTTT250.00332359220.97053518
GCCTTTT303.8351494E-420.97053518
CAGTTTT250.00332359220.97053518
AAATTTT303.8351494E-420.97053518
GGCGTTT303.8351494E-420.97053517
Produced by FastQC (version 0.11.2)