FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_41.3510000005da3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_41.3510000005da3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28555
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC2240.7844510593591316No Hit
TCAACCGATAGGCTGTCTCTTCTACAC680.23813692873402206No Hit
TCAACCGATAGGGCTGTCTCTTATACA460.1610926282612502No Hit
TCAACCGATAGGCCTGTCTCTTATACA420.14708457362983715No Hit
TCAACCGATAGGGGCTGTCTCTTATAC340.11906846436701103No Hit
TCAACCGATAGGAGCTGTCTCTTATAC310.10856242339345122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT25950.021.1269863
CAACCGA25650.021.1269862
CACCGAT751.8189894E-1221.1269843
ACCCGAT506.502705E-821.1269843
TCAACCG25800.021.1269841
TCTACCG506.502705E-821.1269841
CCACCGA751.8189894E-1221.1269842
TCAAACG455.610909E-721.1269841
TAACCGA250.00318682421.1269842
CACCCGA506.502705E-821.1269842
TACCGAT506.502705E-821.1269843
TCCACCG751.8189894E-1221.1269841
TCACCCG506.502705E-821.1269841
TTAACCG250.00318682421.1269841
AAACGAT455.610909E-721.1269843
CAAACGA455.610909E-721.1269842
CTACCGA506.502705E-821.1269842
AGGGGTG354.438297E-520.97898510
AGCCTGT303.8314523E-420.97898518
TGGTTTT354.438297E-520.97898518
Produced by FastQC (version 0.11.2)