FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_4.3510000005d5ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_4.3510000005d5ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30595
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC3931.2845236149697663No Hit
AGACGTCGATACCTGTCTCTTCTACAC1030.3366563163915673No Hit
AGACGTCGATACGCTGTCTCTTATACA330.1078607615623468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCGA454.997237E-721.3913063
GACATCG454.997237E-721.3913062
AGACGTC26300.021.3913061
GACGTCG26300.021.3913062
AGACATC454.997237E-721.3913061
CCGTCGA1750.021.3913043
AGTCGTC751.8189894E-1221.3913041
TCGTCGA751.8189894E-1221.3913043
AGACCTC250.00296882821.3913041
AGCCGTC1750.021.3913041
GACCTCG250.00296882821.3913042
ACGTCGA26550.021.351023
TGGTTTT800.020.9361718
TCCTTTT354.5221568E-520.9361718
TGCATTT354.5221568E-520.9361717
GTGTTTT850.020.9361718
GGGTTTT1400.020.9361718
GCCTTTT354.5221568E-520.9361718
CAGTTTT405.269181E-620.9361718
CGATACC6400.020.936177
Produced by FastQC (version 0.11.2)