FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_38.3510000005d9df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_38.3510000005d9df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16976
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA1110.6538642789820924No Hit
TGTTGCCGAACAGCTGTCTCTTATACA380.22384542884071632No Hit
TGTTGCCGAACACCTGTCTCTTATACA320.1885014137606032No Hit
TGTTGCCGAACAGGGGGGGGTTTTTTT190.11192271442035816No Hit
TGTTGCCGAACATCTGTCTCTTATACA170.10014137606032046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGCC250.00287913321.4350451
TGTTGCC14950.021.4350451
GATGCCG250.00287913321.4350452
TGCTGCC250.00287913321.4350451
GCTGCCG250.00287913321.4350452
ATGCCGA250.00287913321.4350453
CTGCCGA250.00287913321.4350453
TGGTGCC709.094947E-1221.4350451
GTTGCCG14950.021.4350452
TTGCCGA14950.021.4350453
GTGCCGA709.094947E-1221.4350453
GGGTTTT950.020.92920518
TGTTTTT1500.020.92920519
CGAACAT3450.020.9292057
GAACAGC1500.020.9292058
GAACACC751.8189894E-1220.9292058
AACAGCG751.8189894E-1220.9292059
GCCGAAC16650.020.9292055
ACAGCGG303.80349E-420.92920310
ACAGCGA250.003310328320.92920310
Produced by FastQC (version 0.11.2)