FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_37.3510000005d9b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_37.3510000005d9b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20002
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1190.5949405059494051No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC330.164983501649835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA17700.021.227852
CTCTAGT17700.021.227851
CTCTCGT1400.021.2278481
TCTCGTA1400.021.2278482
CTAGTAC17950.021.2278483
CTCGTAC1400.021.2278483
CGACAGC250.003300598120.96250210
TTGTTTT506.801929E-820.96250218
CAGTTTT303.7960772E-420.96250218
ATCTTTT250.003300598120.96250218
AATTTTT751.8189894E-1220.96250219
GTTCTGT250.003300598120.96250220
TTATTTT303.7960772E-420.96250218
TTCTGTC303.7960772E-420.96250221
TGCTGTC303.7960772E-420.96250219
GGATTTT303.7960772E-420.96250218
GGTTTTT2900.020.96250219
CGAAGGT250.003300598120.96250210
CCGTTTT303.7960772E-420.96250218
CGAGGTC303.7960772E-420.96250210
Produced by FastQC (version 0.11.2)