FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_36.3510000005d99c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_36.3510000005d99c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31782
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA2150.6764835441444843No Hit
CAGAACGACAGTGGCTGTCTCTTATAC550.1730539298974262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACGA27800.021.1717012
CAGAACG27800.021.1717011
CAGCACG1350.021.17171
TAACGAC354.1535306E-521.17173
GAACGAC28250.021.17173
ATAACGA354.1535306E-521.17172
CTGAACG455.5379314E-721.17171
GCACGAC1350.021.17173
GACCGAC1300.021.17173
CAGACCG1300.021.17171
AGCACGA1350.021.17172
AGACCGA1300.021.17172
TGAACGA455.5379314E-721.17172
CATAACG354.1535306E-521.17171
ACAGTGG5700.020.9716558
ACAGTCC1350.020.9716558
ACAGTAA850.020.9716558
GACAGTA5300.020.9716557
AGTAGTG405.203161E-620.97165510
CAGTAGT850.020.9716559
Produced by FastQC (version 0.11.2)