FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_35.3510000005d972.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_35.3510000005d972.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52570
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA6671.2687844778390716No Hit
CACAGAAGGACTGCTGTCTCTTATACA570.10842685942552786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA2400.021.1554721
ACCGAAG2400.021.1554722
TCAGAAG900.021.1554722
CTCAGAA900.021.1554721
CCGAAGG2400.021.1554723
ACAAAAG250.003197511421.155472
CACAAAA250.003197511421.155471
ATAGAAG1000.021.155472
CATAGAA1000.021.155471
CACAGAA47200.021.155471
CAAAAGG250.003197511421.155473
CAGAAGG48150.021.1335013
ACAGAAG47250.021.1330832
ACTGCTT250.003361391620.97431210
GGAGATT250.003361391620.97431216
ACTGTTG558.62201E-920.97431210
CTCTGGG303.8972587E-420.97431211
AACGTTT303.8972587E-420.97431217
TCCTTTT250.003361391620.97431218
ACTGCGT601.0168151E-920.97431210
Produced by FastQC (version 0.11.2)