FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_34.3510000005d965.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_34.3510000005d965.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17173
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC1480.8618179700692948No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC620.36103185232632623No Hit
ACTCGTCTCGATGCTGTCTCTTATACA290.1688697373784429No Hit
ACTCGTCTCGATAGCTGTCTCTTATAC240.1397542654166424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC15650.021.2662731
ACCCGTC303.4136802E-421.2662731
CCGTCTC303.4136802E-421.2662733
CCCGTCT303.4136802E-421.2662732
TCGTCTC15750.021.2662733
CTCGTCT15650.021.2662732
ACACGTC250.003016933821.2662721
ACGTCTC250.003016933821.2662723
CACGTCT250.003016933821.2662722
TCGATTG1150.020.956278
TCGATCA557.719791E-920.956278
TCGATAC557.719791E-920.956278
TGATTTT557.719791E-920.9562718
CCTTTTT557.719791E-920.9562719
CGATGGC557.719791E-920.956279
CGATGGA557.719791E-920.956279
CGATGCG557.719791E-920.956279
CGATGCA557.719791E-920.956279
CGATGAC557.719791E-920.956279
CGTCTCG16550.020.956274
Produced by FastQC (version 0.11.2)