FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_33.3510000005d94c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_33.3510000005d94c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20002
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA990.49495050494950504No Hit
AACGGAATGGCTCCTGTCTCTTATACA560.27997200279972No Hit
AACGGAATGGCTGCTGTCTCTTATACA540.26997300269973No Hit
AACGGAATGGCTGTCTCTTATACACAT470.23497650234976503No Hit
AACGGAATGGCTCTGTCTCTTATACAC460.22997700229977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGAA505.2723408E-821.4666651
AACGGAA18450.021.4666651
ATGGAAT505.4189513E-821.4117642
TGGAATG505.4189513E-821.4117643
ACGGAAT18500.021.4117642
CGGAATG18650.021.354363
GGCTGGG1050.020.9300029
GTGTTTT558.034476E-920.9318
GCCTTTT354.4319633E-520.9318
AAATTTT354.4319633E-520.9318
AATTTTT1100.020.9319
ACGTTTT303.8360708E-420.9318
GCGGTTT354.4319633E-520.9317
ATGGCTC4300.020.937
GCTGGGG354.4319633E-520.9310
GCTGGGC303.8360708E-420.9310
TTCTGTC303.8360708E-420.9321
GCTGGCG303.8360708E-420.9310
GCTGGAG303.8360708E-420.9310
GTATTTT303.8360708E-420.9318
Produced by FastQC (version 0.11.2)