FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_32.3510000005d915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_32.3510000005d915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42372
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC2390.5640517322760313No Hit
GGATAGTTAGCCTGTCTCTTATACACA1040.24544510525818936No Hit
GGATAGTTAGCCCCTGTCTCTTATACA470.11092230718398942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT353.8129027E-521.4353081
GGATAGT35550.021.4353081
ATAGTTA35900.021.4353083
CTAGTTA1950.021.4353083
TTAGTTA1500.021.4353083
GATAGTT35550.021.4353082
GATCGTT1800.021.4353072
GGATCGT1800.021.4353071
ATCGTTA1800.021.4353073
AGCCCGA303.9368562E-420.9291639
AGCCTGG1200.020.9291639
AGCCCCT354.58765E-520.9291639
AGCCTCT354.58765E-520.9291639
AGCCTCC303.9368562E-420.9291639
AGCCCAA354.58765E-520.9291639
AGCCTAT558.716597E-920.9291639
TTCATTT303.9368562E-420.92916317
AGCCATT303.9368562E-420.9291639
GCCCAGG354.58765E-520.92916310
AAATTTT303.9368562E-420.92916318
Produced by FastQC (version 0.11.2)