FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_30.3510000005d8d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_30.3510000005d8d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21714
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC1350.6217187068250898No Hit
AGTGTGACGAACCTGTCTCTTCTACAC460.21184489269595652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGACG403.9922506E-621.5558193
GAGTGAC353.5424942E-521.5558192
TGCGACG250.002812022321.5558193
AGGGTGA403.9922506E-621.5558191
GCGTGAC353.5424942E-521.5558192
AGAGTGA353.5424942E-521.5558191
AGTGCGA250.002812022321.5558191
AGTGTGA19400.021.5558191
AGTGACG353.5424942E-521.5558193
GTGCGAC250.002812022321.5558192
CGTGACG353.5424942E-521.5558193
GTGTGAC19400.021.5558192
GGGTGAC403.9922506E-621.5558192
TGTGACG19550.021.5006893
TGGTTTT558.221832E-920.9101418
AACGTGG405.2089254E-620.9101410
AACGTGC405.2089254E-620.9101410
CAGTTTT651.10958354E-1020.9101418
GGCGTTT456.0579623E-720.9101417
AACTGGG405.2089254E-620.9101410
Produced by FastQC (version 0.11.2)