FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_3.3510000005d583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_3.3510000005d583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47834
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC6951.4529414224191997No Hit
GAGGAACAGAGCTGTCTCTTATACACA2090.43692770832462263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCAC3850.021.3649961
AGGCACA3850.021.3649962
TGGAACA659.276846E-1121.3649962
GAGGTAC404.5048055E-621.3649941
GAGGAAC41200.021.3649941
ATGAACA404.5048055E-621.3649942
AGGAACA41200.021.3649942
GATGAAC404.5048055E-621.3649941
AGGTACA404.5048055E-621.3649942
TGAACAG404.5048055E-621.3649943
GGAACAG41800.021.3649943
GGTACAG404.5048055E-621.3649943
GGCACAG3900.021.0910853
TGGTTTT850.020.94037818
ACAGAGT47400.020.9403786
CCAGTTT303.93289E-420.94037817
ACGTTTT601.031367E-920.94037818
GGCGCTT303.93289E-420.94037816
GGACTTT303.93289E-420.94037817
CCGTTTT303.93289E-420.94037818
Produced by FastQC (version 0.11.2)