FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_29.3510000005d8b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_29.3510000005d8b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42305
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCACTGTCTCTTATACAC4080.9644250088642006No Hit
AAGGTCCAAGCCTGTCTCTTATACACA3800.8982389788441082No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG880.208013237206004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCA455.043912E-721.4135442
AGGACCA850.021.4135442
TGTCCAA353.8428985E-521.4135443
GGACCAA850.021.4135443
GGTCCAA39500.021.4135443
AAGGTCC39100.021.4135441
ATGTCCA353.8428985E-521.4135442
AGGTCCA39100.021.4135442
AAGGACC850.021.4135441
AATGTCC353.8428985E-521.4135441
GGCCCAA250.002970042221.4135423
AGGCCCA250.002970042221.4135422
TCCTTTT456.282753E-720.93262518
AGGGGGA250.0033895420.93262512
GCCTTTT507.385461E-820.93262518
ATCTTTT250.0033895420.93262518
AGCTTTT701.4551915E-1120.93262518
GAAATTT250.0033895420.93262517
GCAAGGG250.0033895420.93262510
TGGTATC250.0033895420.93262514
Produced by FastQC (version 0.11.2)