FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_28.3510000005d89d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_28.3510000005d89d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18420
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC2151.1672095548317047No Hit
AGAGGACGAGCTGTCTCTTATACACAT1260.6840390879478827No Hit
AGAGGACGAGATCTGTCTCTTCTACAC630.34201954397394135No Hit
AGAGGACGAGATGCTGTCTCTTATACA410.2225841476655809No Hit
AGAGGACGAGCTGTCTCTTATCCACAT360.19543973941368079No Hit
AGAGGACGAGATCCTGTCTCTTATACA250.13572204125950055No Hit
AGAGGACGAGATGGGGGCTGTCTCTTA240.13029315960912052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGACG15900.021.4512542
AGGACGA16000.021.4512543
AGAGGAC15900.021.4512541
AGTGGAC505.252332E-821.4512541
CGGACGA1150.021.4512543
GCGGACG1150.021.4512542
AGCGGAC1150.021.4512541
TGGACGA505.252332E-821.4512543
GTGGACG505.252332E-821.4512542
ATGGGGG405.102356E-620.92663211
GTGTTTT405.102356E-620.92663218
GGGTTTT405.102356E-620.92663218
GATGTGG405.102356E-620.92663210
TAGTTTT405.102356E-620.92663218
GCGTTTT800.020.92663218
AGATGCT405.102356E-620.9266329
AGATGAC405.102356E-620.9266329
CGAGATC4150.020.9266327
CGAGATA3200.020.9266327
AGTTTTT950.020.92663219
Produced by FastQC (version 0.11.2)