FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_25.3510000005d84d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_25.3510000005d84d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18619
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC430.23094688221709006No Hit
CTTGGTTCGTTGCTGTCTCTTCTACAC210.11278801224555561No Hit
CTTGGTTCGTTGGCTGTCTCTTATACA190.10204629679359795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG16700.021.3369873
GGGTTCG556.340997E-921.3369873
CTTGGTT16400.021.3369871
TTGGTTC16450.021.3369872
TGGGTTC556.340997E-921.3369872
CTTGATT505.571019E-821.3369871
TTCGTTG18400.020.9919156
GGTTCGT17800.020.9919154
ATTCGTT506.634946E-820.9919155
GTGTTTT557.918061E-920.93548418
GGGTTTT1150.020.93548418
GTTGAAG250.003316662320.9354849
AATTTTT1350.020.93548419
ACGTTTT455.892216E-720.93548418
TTGTAGC250.003316662320.93548410
CGCTTTT455.892216E-720.93548418
GCGGTTT250.003316662320.93548417
ATGGTTT250.003316662320.93548417
TTCTGTC250.003316662320.93548421
GGATTTT751.8189894E-1220.93548418
Produced by FastQC (version 0.11.2)