FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate4_24.3510000005d823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate4_24.3510000005d823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27175
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC3291.2106715731370745No Hit
GTGTAGTGTGGCTGTCTCTTATACACA2961.0892364305427784No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC1270.46734130634774607No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC1090.4011039558417663No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC1050.38638454461821525No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA880.32382704691812325No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC750.27598896044158233No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC730.2686292548298068No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC710.2612695492180313No Hit
GTGTAGTGTGGACCTGTCTCTTATACA670.24655013799448022No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC620.2281508739650414No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC430.15823367065317387No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT420.1545538178472861No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC370.1361545538178473No Hit
GTGTAGTGTGGATCTGTCTCTTATACA360.13247470101195952No Hit
GTGTAGTGTGGATACTGTCTCTTATAC350.12879484820607176No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC350.12879484820607176No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC330.12143514259429623No Hit
GTGTAGTGTGGACTCTGTCTCTTATAC280.10303587856485741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGT1150.021.3033711
GTCGTGT1150.021.3033713
GGTAGTG353.9306513E-521.3033712
TGTCGTG1150.021.3033712
GTAGTGT24900.021.3033713
GTGTAGT24000.021.303371
CGTAGTG455.155998E-721.303372
TGTAGTG24000.021.303372
GATGGGG250.003342270920.95027711
GGATGTG250.003342270920.95027710
AGGGGGG303.8602637E-420.95027712
GGAGAGG303.8602637E-420.95027710
GGAGAGC303.8602637E-420.95027710
GGATGGG558.274583E-920.95027710
GGCATTT303.8602637E-420.95027717
TAGTGTG25600.020.9502774
GAGTGGT303.8602637E-420.95027711
GAGTGAG250.003342270920.95027711
GTTCTTT250.003342270920.95027717
TTATTTT405.202859E-620.95027718
Produced by FastQC (version 0.11.2)